A modified DNPH derivatization method in RPLC for quantification and determination of dihydroxyacetone and formic acid in formose samples

Zafar Iqbal

Abstract

Dihydroxyacetone (DHA) and formic acid (HCOOH) in formose samples were analyzed using reversed-phase liquid chromatography (RPLC) utilizing amodified chromatographic and 2, 4-Dinitrophynylhydrazine (DNPH)- derivatization techniques. Especial evaporation technique was developed to remove excess of unconverted substance from the formose mixture to facilitate the reduction of excess of reagent used for derivatization. Usage of derivatization reagent (DNPH) was reduced by a factor of 60. The optimumconditions for separation of DHA-hydrazone were (5-90)%ACN at 1 ml/min. Sodiumborohydride (NaBH4) reduction of aldehydes, ketones and sugars helped to analyze HCOOH-hydrazone quantitatively in formose sample.