Research Article
Barrios-Ramos JP, Garduno-S
Abstract
Obesity, dyslipidemia, and glucose utilization disorders are the main indicators in the diagnosis of metabolic syndrome. However, there are other markers of damage from oxidative stress, such as the LDL cholesterol (LDL-c) plasma concentration, protein oxidation, lipoperoxidation, and DNA damage. In this study, male Wistar rats (250-270 g) were used. The rats were distributed into eight groups as follows: 1) standard diet and purified water intragastrically (1ml/kg) for seven weeks (N); 2, 3, 4, 5, 6 and 7) hypercholesterolemic diet and 60% fructose for four days (4D);one (1W),two (2W); three (3W), four (4W), five (5W), six (6W) and 7 (7W) weeks respectively and 8) 60% fructose for four weeks (4WF). At the end of treatment, the concentrations of total cholesterol (TC), HDL cholesterol (HDL-c), triglycerides (TGs), and glucose, as well as the alkaline phosphatase activity (ALP), were determined. Moreover, the percentage of cells with DNA migration was determined with a comet assay, and a histopathological study of the hepatic samples was conducted. The animal model in the current study showed metabolic syndrome (MS) in animals after four weeks with the following well defined markers: decreased HDL-c; increased total cholesterol, LDL-c, atherogenic index, weight gain, and liver weight; hypertension; and defined steatosis leading to an increase in plasma ALP and genotoxic liver damage.