A Versatile Metagenome Purification Method to Identify Uncultivable Bacteria by Denaturing Gradient Gel Electrophoresis (DGGE) from\r\nSediments and Soils

Lourdes Reyes-Escogido, Ingrid

Abstract

Here, we report a versatile metagenome purification method to identify uncultivable species from sediments and soils by nested-PCR-DGGE according to 16S rDNA. This combination of methods uses enzymatic lysis in situ, polyvinylpyrrolidone (PVP), Chelex 100, glass bead-silica gel and chaotropic salts, with the advantage that it can be applied to different soils.