Research Article
Cheng-Chih Tsai, Lan-Chun C
Abstract
Objectives: The aim of this study was to investigate the conditions for viable lactic acid bacteria (LAB) cells and their heat-killed LAB, and the formulation as well as an alternative treatment to antibiotics to reduce infection, invasion and the inhibition of IL-8 secretion induced by S. choleraesuis for HT-29 cells. Methods: To examined the efficacy of live LAB and heat-killed products in (1) inhibiting Salmonella choleraesuis growth by the plate agar-well diffusion assay, (2) adhering to swine intestinal cells and human intestinal Caco-2 cell line, (3) inhibiting the invasion of HT-29 cell line by Salmonella choleraesuis, and (4) inhibiting Salmonella choleraesuis induced interleukin (IL)-8 secretion by HT-29 cells Results: The results indicated that viable LAB and heat-killed products significantly reduced S. choleraesuis infection, invasion in the HT-29 cell line, and had the adhesion ability of the LAB to the swine intestinal cells and human intestinal Caco-2 cell line. Both viable and heat-killed LAB powder possess an ability to reduce the S. choleraesuis induced interleukin (IL)-8 secretion by HT-29 cells, and viable bacteria powder products are the most effective. Conclusion: Both viable and heat-killed LAB powder products were potentially useful for the protection of the epithelial cells against Salmonella infection, invasion and the induced inflammation for swine and human.