An HPLC method for the determination of busulfan in biological samples

M.A.Navarro Moreno, A.Moreno G

Abstract

An HPLC analytical method for assessing plasma busulfan rat has been developed. Thismethod uses 1,5-Bis (metansulfoniloxy) pentane, as internal standard, dimethylformamide (DMF) as solvent, Tri Butyl n Phosphine as an antioxidant and 8Mercaptoquinoline (Q) as a reagent.We have evaluated the accuracy, reproducibility and speed, with satisfactory results.