Antioxidant Activities and Thin Layer Chromatographic Analysis of Aqueous Extract of Barks of Cinnamomum zeylanicum Blume.

C. Sivaraj, K. Abirami, T. K.

Abstract

Objective: The objective of the present study was to investigate phytochemical constituents, free radical scavenging activities and TLC of the aqueous extract of barks of Cinnamomum zeylanicum Blume. Methodology: The qualitative and quantitative analyses were carried out for the aqueous extract of barks of C. zeylanicum using standard procedures. The antioxidant activities were carried out by DPPH, ABTS•+, OH• and NO• radical scavenging assay as well as Fe3+ reducing power and phosphomolybdenum reduction assay methods. The aqueous extract of barks of C. zeylanicum showed good radical scavenging as well as reducing power activities. Results: The Phytochemical estimation revealed that the concentration of phenols and flavonoids in the aqueous extract of barks of C. zeylanicum were 42.3 ± 2.96 mg/g and 5.81 ± 0.40 mg/g respectively. The maximum percentage of DPPH•, OH•, ABTS•+ and NO• radicals inhibition were 84.39 ± 5.90, 54.84 ± 3.83, 67.06 ± 4.69, and 55.61 ± 3.89 respectively. Conclusion: The results revealed that the aqueous extract of barks of C. zeylanicum significantly inhibits the radicals and the antioxidant activity increases with increasing concentration.

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