Bio-energy production from glucose at various Temperatures from Citrus sinnesis by Trichoderma reesei

J.A. Yabefa, B.O. Aderemi, D.

Abstract

Trichoderma reesei was cultured to secret enzymes using orange mesocarp as subsrate. Untreated orange mesocarp (non-alkali treated orange mesocarp washed with distilled water) and orange mesocarp alkali-treated at 100 ⁰C, washed with water at pH of 6-7.When untreated orange mesocarp and alkali-treated orange mesocarp was hydrolysed at varying temperatures of 30⁰ C,37 ⁰C, 40 ⁰C,50 ⁰C 55 ⁰C, appreciable glucose yield was found(2.13, 2.34, 1.71, 2.56, 2.35) mmol/L respectively for alkali –treated mesocarp, but the yield was lower when untreated mesocarp was hydrolysed at 37 ⁰C(1.7mmol/L).Hence it was concluded for this particular research that untreated orange mesocarp is as good a substrate for glucose production but the optimum temperature for the alkali-treated mesocarp falls within 37-50 ⁰C.