Biomimetic 3D bioprinting of cellular laden nanocomposite scaffold through co-axial and core-co-cultured structure

Fahimeh Shahabipour


There is a need to recapitulate the native complexity of bone structure within engineered 3D structures with tailored bi­ological and mechanical properties. In this study, we sug­gest an innovative cell-printing process, supplemented with core/shell nozzle and co-cultured/mono-cultured methods, to achieve 3D osteon-like structures through cell-laden bio­inks using an extrusion-based 3D bioprinter in one-step. In this study, vascularization promoting and osteogenic bioinks were developed based on different concentration of Gel­MA-alginate hydrogels with the incorporation of hydroxy­apatite nanoparticles. These hydrogels were chosen due to their suitable mechanical stability, swelling ratio, and print­ability. To obtain a core/shell osteon-like structure (CSBP), we used a vascularization bioink combined HUVECs in the core region, and used osteogenic- MC3T3-E1 cells-laden bioinks in the shell region. Pure gelatin was concentration in all bioinks to support both of core and shell structures during 3D bioprinting. Core-co-cultured osteon-like struc­ture (CCBP) was fabricated through co-culturing of HU­VECs and MC3T3 cells within bioink in the core region. Mono-cultured printed structure composed of single cell lines served as a control. The fabricated 3D-core-cocultured of HUVECs-MC3T3 cells showed significantly higher cell viability (84%) compared to that (78%) of a 3D-core/shell of HUVECs/MC3T3 cells. Both fabricated structures exhib­ited outstanding cell viability in comparison with (65%) of mono-cultured 3D cell-laden scaffold (control). In addition, significant increases in osteogenic properties were observed in the co-culture samples versus the mono-culture controls. We demonstrated that both co-culture configurations were able to promote mineral deposition in the absence of exog­enous osteogenic factors. Although the CSBP configuration displayed less viability than CCBP, this structure still exhib­ited good osteogenic and angiogenic properties. In conclu­sion, this investigation provided highlighted the potential of both structures as biomimetic bone scaffolds for complex bone tissue and other tissue engineering application.

Relevant Publications in European Journal of Applied Engineering and Scientific Research