Callus induction and in vivo and in vitro comparative study of primary metabolites of Withania Somnifera

Satyapal Singh; Babeet Singh T

Abstract

In present study callus was raised from the leaf explant of Withania somnifera. Maximum callus was obtained on MS medium supplemented with 2, 4- D 1.0 mg/liter and IBA 0.5 mg/liter. The callus and different plant parts were used for primary metabolite quantification and antioxidant activity. Maximum soluble sugars found in callus, maximum amount of starch, protein and phenolic contents were found in stem and maximum lipid found in leaf.

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