Research Article
Sasan Hamzei,Milad Anvarian,Ma
Abstract
Lactobacilli bacteria are gram positive non-spore forming, catalase-negative and filamentous bacteria that mainly ferment different carbohydrates into lactate and acetate. Fifty perch (perca fluviatilis) hunted from Aras Dam, Iran, randomly. The samples were transported to the Microbiology Laboratory, under sterile conditions in the vicinity of ice and after dissection according to sterile method 1 gram of stool was sampled from the anterior part of intestine and cultured in MRS agar-plates. After phonotypical and biochemical identity of bacteria, for distinction of lactobacillus species molecular characterization, 16S rDNA gene was amplified and PCR products were sequenced. Results of sequencing about 3 isolated bacteria indicated that this species has been Lactobacillus plantarum and the founding’s corresponding to produce results from biochemical tests and determine cutting pattern for this species.