Chlorambucil inclusion complexes studywith -cyclodextrins through NMR, IRspectroscopy, x-ray crystallography and capillary electrophoresis

Miguel A.HernÃ

Abstract

The capacity of cyclodextrins to house molecules such as chlorambucil was studied by 1H and 13C NMR through inclusion complexes formed from chlorambucilwith three -cyclodextrins; in addition, infrared spectroscopy, X-ray crystallography and capillary electrophoresis studieswere conducted. Solid complexes were obtained by co-precipitationmethod, with 1H and 13C homonuclear studies, 2D techniques (COSY, HETCOR and ROESY) of 1H- 13C heteronuclear correlation of liquid-phase nuclear magnetic resonance and solid-phase for 13C. The results demonstrate the formation of inclusion complexes, their stability in aqueous solution, and provide evidence to identify the interactions between chlorambucil and the studied cyclodextrins.