Research Article
Fozia Anjum, Shazia Anwer Bukh
Abstract
Extracts of Cuscuta stem collected over different hosts were prepared by stimulated effect of ultrasonic waves using different solvents like 100% methanol, 80% methanol, 100% ethanol, 80% ethanol, water and n-hexane. Maximum extract yield (g/100 g of DW) was detected by using 80% methanol solvent (11.72-26.14) followed by water (10.52-22.95), 80% ethanol (10.19-22.63), methanol (8.91-22.61), ethanol (5.92-15.12), and n-hexane (3.82-8.72). Maximum level of TPC (71.11), TFC (85.11), reducing power (2.56), DPPH scavenging activity (59.57), percent inhibition of linoleic acid peroxidation (87.49) and δ-tocopherol (100 μg of δ -Toc/g of FW) was found significantly higher (P < 0.05) in stem collected from Z. jojoba whereas α-(21 μg of δ -Toc/g of FW) and ϒ-tocopherol (96 μg of δ -Toc/g of FW) contents were found higher (P < 0.05) in E. jambulana and C. latifolia. A strong correlation between total phenolics (R2=0.916) or reducing power (R2=0.561) and antioxidant activity was found in methanolic extract whereas flavonoids exhibited weak correlation with antioxidant activity.