Direct Comparison of DNA versus mRNA on the Efficiency of Induced Pluripotent Stem Cells Generation from Human Primary Fibroblasts

Sandra Aurora Telpalo- Carpio,

Abstract

Induction of pluripotency in somatic cells has opened a brand new spectrum on patient-specific stem cell therapies, therefore several approaches seek the optimization of the existing dedifferentiation processes. DNA and mRNA have been used to induce cells using different strategies, from diverse sets of pluripotency induction genes to variations of their delivery method. In this work we present a direct comparison between circular and linear DNA, as well as mRNA, capability to dedifferentiate human primary fibroblasts.