Elemental analysis and Anti-microbial potentials of the leaf extract of Cassia arereh Del.

Adeyanju Olusola, Olajide Olut

Abstract

The phytochemical analysis, elemental analysis, antimicrobial activities and the minimum inhibitory concentration (MIC) of aqueous and ethanolic extracts of the leaves of Cassia arerehDel. were carried out. Phytochemical investigation revealed the presence of several bioactive compounds such as alkaloids, flavonoids, anthraquinones, saponins, glycosides, tannins, carbohydrates and terpenes. Elemental analysis revealed the presence of calcium (Ca), phosphorus (P), Manganese (Mg), Manganese (Mn), Iron (Fe), Copper (Cu) and Zinc (Zn).Manganese was present at a higher concentration (11.50 µg/g) followed by Zinc (3.35 µg/g). Antimicrobial studies showed that the ethanolic extract had considerable inhibitory activity against Staphylococcus aureus, Streptococcus pyogen, Salmonella typhi, Shigella dysenteryand Eschenchia coli. Ethanolic extract had higher zone of inhibition (32 mm) againstsalmonella typhi at (0.5 µg/ml) with minimum inhibitory concentration (MIC) value of 8 x 102µg/ml. The MIC ranges from 4 x 102 µg/ml to 5 x 104 µg/ml for other bacteria.

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