Research Article
Nguyen Hoang Loc, Nguyen Thi Q
Abstract
We cloned a gene coding chitinase (chi42) from strains Trichoderma asperellum SH 16 isolated from Thua Thien Hue province, Vietnam. Expression of the chitinase in Saccharomyces cerevisiae INVSc1 produced a fusion protein of approximately 50 kDa (42 kDa of mature chitinase, 4 kDa of signal peptide and 3.4 kDa of fusion tag of pYES2/NT(C)/chi42 vector). The effect of induction time on the chitinase production was investigated. The results showed that the highest total activity (0.012 unit/mL) of chitinase occurred after 12 h of induction with 2% galactose.