Fingerprinting of Natural Product by Eastern Blotting Using Monoclonal Antibodies

Yukihiro Shoyama

Abstract

In the recent rapid development of the molecular biosciences and their biotechnological applications, immunoassay systems using monoclonal antibody (MAb) against drugs and small molecular weight bioactive compounds have become an important tool for studies on receptor binding analysis, enzyme assay, and quantitative and/or qualitative analytical techniques in animals or plants; owing to their specific affinity. Previously we prepared various kinds of MAb against natural products like forskolin, solamargine, crocin, marihuana compound, opium alkaloids, ginsenosides, berberine, sennosides, paeoniflorin, glycyrrhizin, ginkgolic acid, aconitine alkaloid, baicalin, and so on, and developed individual competitive enzyme-linked immunosorbent assay (ELISA) as a high sensitive, specific, and simple methodology.