High-performance thin-layer chromatographic method for quantification of -sitosterol from Tridax procumbens

Shaikh Shamshul S., K.V.Mangao

Abstract

A simple, precise, accurate and reproducible high-performance thin layer chromatographic method has been established for quantification of -sitosterol in whole plant powder of Tridax Procumbens. The amount of - sitosterol in whole plant powder of Tridax Procumbens was found to be 0.04%. A methanolic extract of the whole plant powder was used for the experimental work. Sepration was performed on aluminiumsheet silica gel 60F254HPTLCplateswithToluene:Ethyl acetate:Glacial acetic acid 8.0:2.0:0.2 (v/v), as solvent system. After development, plates were treated with Anisaldehyde Sulphuric Acid reagent. Detection and quantification were performed by densitometer at = 550nm. -sitosterol response was linear over the range 10.0g/ml to 70.0g/ml. The validatedHPTLCmethod can be used for a routine quality-control analysis of Tridax Procumbens whole plant powder and quantification of -sitosterol.