The presence of nanoparticles in waste matter water has raised considerations regarding the accumulative toxicity of municipal effluent to aquatic organisms. The aim of this study was to look at the consequences of dietary flowers of zinc nanoparticles (nano-ZnO) and ZnCl2 (at a hundred metric weight unit total Zn/g food) in adult twat minnows Pimephales promelas throughout continuous exposure to a physico-chemically treated effluent for twenty one days. Immunocompetence determined by assessing white blood corpuscle viability, bodily process activity, aerophilic stress (lipid peroxidation) and DNA strand breaks in gills. The results discovered that white blood corpuscle viability attenuated with increasing effluent concentration, whereas it magnified somewhat in fish fed either kind of zinc. The decrease in viability wasn't discovered in fish exposed to the municipal effluent that were fed either kind of zinc. bodily process activity attenuated when Associate in Nursing initial increase at an occasional concentration of the effluent (5% v/v), whereas it pronto attenuated in fish fed either kind of zinc. The decrease was conjointly discovered in fish fed either kind of zinc that were exposed to the effluent. the information discovered that nano-ZnO toxicity differed from ZnCl2 effects, however once the fish were each exposed to the effluent and fed a nano-ZnOcontaminated diet, the effects closely resembled the consequences in fish fed a ZnCl2-supplemented diet. lastly, eaten nanoparticles in food might have an effect on the system of fish exposed to municipal wastewaters otherwise than non-exposed fish, rendering the exposed fish a lot of susceptible to microorganisms.