Fernanda Marques Peixoto, Rono
Abstract
Increasing â-carotene content from food is not a guarantee of combat micronutrients deficiencies, such as hidden hunger, because there are many aspects involved in absorption and metabolization by human body. Therefore, in vitro bioaccessibility studies have been used as a prediction tool of understanding food matrix factors that may cause release to its absorption. These studies are conducted by applying in vitroﾃ窶堙や digestion methods which expose lineage /micronutrient to human physiological conditions, bymimicking oral, gastric and intestinal digestion human. This work aimed to implement ﾃ窶堙や亙n vitroﾃ窶堙や digestion methodology as tool to determinate ﾃ窶堙や亙n vitroﾃ窶堙や bioaccessibility from improved cultivars with higher levels of ﾃδｯﾃつﾃつ｢- carotene. Analyses involved enzymes as á-amylase, pepsin, bile, pancreatin, lipase and mucin; and inorganic compounds such as KCl, KSCN,NaH2PO4,Na3PO4,NaOH,NaCl,CaCl2,HCl,NaHCO3. Physiological variations were reproduced by the heating bath shaker with orbital gyrus (37ºC) and centrifugation (5000g, 45 min). Quantification and determination of the carotenoids profile were performed by high performance liquid chromatography (HPLC)withYCM®C30CarotenoidS-3 4.6 x 250mmcolumn and UV-Vis spectrometer. Therefore, thismethodology proved to be faster and cheaper, inasmuch as in vivo studies are more costly, complex and requiremore time.