Influence of Inoculum age and Inoculum ratio on the protease production from Bacillus subtilis isolated from soil sample

Ela Tiwari and Shivangi Thakur

Abstract

Bacillus sp being industrially important organisms produces a wide variety of extra-cellular enzymes including proteases. Bacillus sp isolated from local from soil samples collected from Ghadi soap industry, Chanatoria, Sagar, M.P., India and identified as Bacillus subtilis. The isolated strain produced alkaline protease at the best inoculums age and inoculums ratio of 30 hr and 1ml-1 respectively.