Isolation and Elucidation of Triterpenoid and Flavonoid Pure Compounds in Morus alba Stem Bark

D Rosa, N Yuliana, YH Choi and

Abstract

Two pure compounds, morusin (a flavonoid, known for its bioactivity as tumor inhibitor, anti-bacterial, and anti-inflammatory agents) and betulinic acid (a triterpenoid, known for its anti-tumor activity), are isolated and elucidated from extract of Morus alba stem bark. Column chromatography and thin-layer chromatography techniques are used for the isolation, and nuclear magnetic resonance (NMR) analysis, both 1D and 2D, is used to elucidate the structure of the pure compounds.