L-Glutaminase Production by Aspergillus Wentii Mtcc 1901 under Solid State Fermentation using Mixed Agro Industrial Residues

B. Revanth and K. Jaya Raju

Abstract

Optimization of physicochemical parameters for production of extracellular L-glutaminase using Aspergillus wentii MTCC 1901 under solid state fermentation is detailed. Substrate screening showed significant results for wheat bran, sesame oil cake (SOC) and green gram husk (GGH). Furthermore, 1 : 1 combination of SOC and GGH showed best results among six different mixed substrate combinations performed. The maximum activity (259.32 U/gd s) of L-glutaminase by Aspergillus wentii MTCC 1901 was obtained using SOC : GGH (1 : 1) of 40% initial moisture content, initial pH 8.0, supplemented with sucrose (4.0% w/v), peptone (2.0% w/v), magnesium sulphate (1% w/v) and L-glutamine (3.0% w/v), inoculated with 2 mL of 96 hours old fungal culture and incubated at 32oC for 96 hours. Both physical and chemical parameters had played a significant role in the production of L-glutaminase. Medium with optimized conditions and supplementation gave a maximum glutaminase activity of 259.32 U/gds, which is above 6 fold increase in activity than that with medium of basal conditions (39.9 U/gds). An antileukemic agent L-glutaminase with relatively good activity is produced from agro industrial residues in a mixed substrate combination.