Research Article
Nyarko H. D, Obodai E. A, Boam
Abstract
This research was conducted at three fish smoking sites and three marketing centres in the Tema municipality, to compare the microbial load of smoked sardine (Sardinella aurita). Microbial load of smoked sardine were determined using aerobic plate count, yeast and moulds, coliform bacteria, Escherichia coli and Staphylococcus aureus counts, Salmonella typhi detections, with pH and water activity. The results indicated that the aerobic plate counts within the smoking sites ranged from 6.2 x 104 to 3.3 x 105 cfu/g; yeast and moulds from 1.1 x 102 to 9.3 x104 cfu/g; and coliform bacteria from 0.0 to 2.1 x 104 cfu/g. For marketing centres, the values ranged from 7.2 x 104 to 4.1 x 107 cfu/g, 5.0 x 102 to 8.0 x 104 cfu/g, and 4.7 to 2.0 x 102 cfu/g for aerobic plate count, yeast and moulds and coliform bacteria respectively . E. coli was poorly represented in samples obtained from Manhean (3.1 x 102 cfu/g) and Newtown (1.8 x104 cfu/g). Staphylococcus aureus was completely absent from all samples analysed. An isolated case of Salmonella typhi detection was made from Community 1 market samples. pH values were between 5.84 and 6.42 for all samples. Microbial counts of smoked sardine samples from smoking sites and marketing centres were significantly different (P < 0.05), with the latter recording higher values. Generally microbial counts for samples from the smoking sites were within acceptable limits of GSB, while those from marketing centres were not.