Partial purification and optimization of xylanase from Bacillus circulans

Dhruti Pithadiya, Deep Nandha,

Abstract

Bacillus circulans was isolated for xylanase production from paper effluent collected from paper mill. Extracellular Xylanase production was observed in the culture medium with pH 7 at 30 ̊ C. Culture medium amended with 1% birch wood xylanwas found to be suitable source to enhance Xylanase yield in shaking condition at 7 pH at room temperature in submerged fermentation. Partial purification of Xylanase was done from crude enzyme extract by three steps (ammonium sulphate precipitation, TCA-Acetone precipitation and Ion exchange chromatography).The molecular mass of Xylanase was ~ 20 KDa and was observed by SDS-PAGE Zymography technique. Optimum Enzyme activity was recorded at 30°C in standard conditions.