Preparation of high purity analytical standards using high performance liquid chromatography in analytical scale

Sidney Pacheco, Ronoel Luiz de

Abstract

Obtaining standards with high purity and reliability is certainly the greatest difficulty and source of errors in analytical chemistry. This study aimed to demonstrate the feasibility of obtaining substances with high purity and in sufficient quantities for use as standards in analytical high performance liquid chromatography (HPLC), in a quickly and practicalway. Standards were isolated from three classes of substances of interest in the food industry (isoflavones, anthocyanins and carotenoids) fromnatural sources, in sufficient quantities to build external calibration curves within the range of work required.