Research Article
Naser Samadi, Reza Ansari, Bak
Abstract
A cheap and simple and precise method was developed and validated for determining of trace Hg2+ ions in real samples. The method was based on the reaction of Hg2+ ions with the novel reagent modified poly (styrene alternative maleic anhydride (SMA)) with melamine (C3H6N3)(M)and melamine-propanedioic acid (C6H8N6O3) (MA)and melamine-thio propanedioic acid(C6H8N6O2S) and after completing the adsorption the mixture was filtered and mercury(II) ions concentration was determined in filtrate after adding KI, NH3 and KOH by UV the formed complex (K2HgI4) is pallid yellow but in presence ammonia is deep yellow shows an absorption maximum at λmax=350 nm in borate buffer of pH = 9.6. The corrected absorbance of the formed complex at λmax was gotten employing β-correction spectrophotometric method. Beer’s–Lambert law of the colored Hg– reagent complex was obeyed in the concentration range of 0.05– 20μgmL−1 Hg2+ ions, with a relative standard deviation in the range of 1.2±0.78%. The detection limits (D.L) and quantification limits (Q.L) of the method were 0.012 and 0.040μgmL−1 Hg2+, respectively. The method is sensitive (ε = 8.37×104 Lmol−1 cm−1), cheap and less toxic than most of the reported extractive spectrophotometric methods .The validation of the method was examined by comparison with the data gotten by the inductively coupled plasma-mass spectrometry (ICP-MS). The statistical procedure of data in terms of Student’s t-tests and variance ratio f-tests has allocated no significant differences.