Sensitive visible spectrophotometric methods for the determination of anagrelide in pure and pharmaceutical dosage forms

Venugopal Veldi, Ramu Golkonda

Abstract

Two simple and sensitive visible spectrophotometric methods (A and B) have been developed for the determination of anagrelide in pure and pharmaceutical dosage forms. Method A is based on the oxidation of 3-methyl benzothiazololinone-2-hydrazone (MBTH) by ferric chloride followed by its coupling with anagrelide in acidic medium forming a dark green colored chromogen with an absorption maximum at 618 nm. Method B is based on the reaction of anagrelide with Folin-Ciocateu(FC) reagent under alkaline conditions forms an intensive blue colored chromogen with an absorption maximum at 685 nm. Beer’s law is obeyed in the concentration range of 5-30 μg/ml for method A and 10-70 μg/ml for method B. These methods were successfully applied to pharmaceutical dosage forms and no interference was observed from common pharmaceutical excipients and additives.  

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