Research Article
Ahombo Gabriel*, Baloki Ngoulo
Abstract
In order to demonstrate the transmission of resistance genes between clinical and community Pseudomonas, 61 strains of Pseudomonas including 47 (77.04%) community Pseudomonas and 14 (22.96%) clinical Pseudomonas were tested with antibiotics by the method of diffusion of the antibiotic discs in solid medium on Mueller Hinton medium. Thirty four strains of DNA comprising 20 (58.82%) community strains and 14 (41.18%) clinical strains exhibiting colistin resistance were extracted; then, the mcr-1 resistance gene was detected by PCR. The antibiotic sensitivity test showed that tobramycin and ciprofloxacin were more active in community and clinical Pseudomonas. The difference was significant with one p<0.05 for five antibiotics between community and clinical Pseudomonas. PCRs of the mcr-1 gene are positive for 8 (40%) community Pseudomonas strains and 5 (35.71%) clinical Pseudomonas strains. The amplified mcr-1 gene fragments were different in size in clinical Pseudomonas.