Study the Immunomodulatory Effects of Combined Extracts of Sesbania grandiflora Flowers and Cocculus hirsutus Leaves on the Circulating Antibody Response

Mallik Arunabha and Nayak Sati

Abstract

Polyherbal therapy is said to be a current pharmacological principle having the advantage of producing maximum therapeutic efficacy with minimum side effects. We assessed the Immunomodulatory effects of a combination of extracts from Sesbania grandiflora flowers and Cocculus hirsutus leaves. In this work we detected the IgM and IgG levels in sera from mice treated with the HP-I, HP-II and HP-III against SRBC in ELISA methods at 0, 4, 7 and 14 days. In addition, we analyzed the phytochemical properties of the different herbal preparations. After oral administration of the dose of combined extract (100-400 mg/ kg b.w.) increase the serum antibody titers after four and seven days, suggesting immunostimulatory activity through modulation of B lymphocyte functions. These results suggested that HP-I from 1:1 of both plants contained several chemical compounds that possess positive modulator effects on the immune system.