Subtractive Imaging Using Gaussian Fits and Image Moments in Confocal Microscopy

Matthew D McCluskey

Abstract

A subtractive imaging method is described that uses Gaussian fits and image moments in the CCD confocal microscopy configuration. Subtraction of the 0th order image moment of a digital pinhole filtered signal from the Gaussian fitted intensity signal reveals missing details of a high resolution test target. By choosing an appropriate weighting coefficient, the associated halo effect can be removed while retaining the fine details.

Relevant Publications in Research Journal of Optics and Photonics