Mohamed A. Shreadah, Nehad
The Ameliorative Role of a Marine Sponge Extract against Mixture of Persistent Organic Pollutants induced Changes in Hematological Parameters in Mice Persistent organic pollutants (POPs), especially polychlorinated biphenyls (PCBs) and organochlorine pesticides (OCPs) are highly toxic to the marine environment. Since little is known about the effect of natural life mixture of POPs on the hematological parameters in Mice, the present study investigated the effect of POPs compounds to induce toxicity in mice using hematological parameter as biomarkers .The protective role of Hyrtios aff. Erectus sponge extract isolated from red sea was also examined. A mixture of PCB 28, PCB 52, , PCB 101, PCB 118, PCB 153, PCB 138 and PCB 180,alpha-Hexachlorocyclohexane, beta-Hexachlorocyclohexane, gamma-hexachlorocyclohexane, Aldrin , O,P’-DDE, Dieldrin, P,p DDE, O,P DDD, Endrin, P,p DDD and P,pDDT were extracted from sediments collected from Lake Mariout. Mice groups were assign into four groups and hematological parameters were compared between these different groups. Total white blood cell (WBC) count, red blood cells (RBC), hemoglobin, and hematocrit were decreased significantly in the induced group. No significant increase were observed for MCV, HCH, MCHC for induction group; while a significant increase in WBC, RBC, and PLT was observed in the pretreated group. On the other hand, a little or almost no change was observed in the pre-treated group by Hyrtios aff. Erectus extract. These findings were confirmed with immunohistochemistry results which showed marked eosinophilia of hepatocyte cytoplasm. At the same time the centrilobular showed hypertrophy and midzonal cytoplasmic vacuolization of hepatocytes in contrast to the pretreated group and the extract group. The present study concluded the existence of significant associations between POPs induce toxicity and hematological different parameters, i.e. WBC, RBC, HCT and PLT. Also the present results confirmed the protective role of Hyrtios aff. Erectus extract which harbor a great diversity of bioactive compounds and minerals.