Research Article
Emanual Michael Patelia and
Abstract
The macrophages especially the macrophages associated with the tumour development are the main focus for the experimental purpose. During the experiment, the RAW 264.7 mouse macrophage cell linewascultured in aseptic conditions and then polarized into the M1 and M2 phenotypes. The cytokines such as IL-4, IFN-γ, and IL-4 were used for the induction of polarization. The expected cellular markers of M2 such as CD204 and CD206 has been shown by Western blot analysis. The M1 and M2 phenotypes were evaluated for phagocyticactivity, M2 phenotype being recorded with highest relative phagocytic activity. The chemo tactic movement of RAW264.7 macrophages kept in co-cultre with B16F10 mouse melanoma cells was similar to the transmigration obtained by RANTES (CCL5) alone. The α-RANTES antibody and the CCR2 antagonist significantly reduced transmigration of macrophages in co-culture with B16F10 cells.