Research Article
Forutan Salehi Nezhad, Akram Y
Abstract
Testis is an organ that its natural function depends on temperature. It was determined that heat stress in male reproductive system followed by at leasttwo negative results. One of them is the exposure of oxidative reaction and the other one is impairment in DNA structure. Thus the present study was conducted aiming at evaluating oxidative reaction indicators and the exposure of DNA damages by using TUNEL method in Sertoli cells under heat stress at 42 in order to determine how heat stress can affect Sertoli cells and study the sort of resulted damages in these cells under these conditions, for this testes of 9 male lambs of 3-10 months were provided from slaughter house and the separation and culture of Sertoli cells were performed on these samples. To evaluate heat stress effect, Petry Dishes containing Sertoli cells were divided into 3 categories; first category was control group and was incubated at 32 . Second category which were under mild thermal stress at 39 centigrade for 6 hours and the third category were under severe thermal stress at 42 centigrade for 6 hours. In order to account cells which are involved in planned death, colored TUNEL was done on three positive, negative control group and test. Four parameters related to oxidative reaction in control group and affected groups under thermal stress were conducted in peroxidation- lipids test via measuring MDA concentration (TBARS),measuring the activity of super-oxide-dismothase enzyme, measuring nitric oxide quantity and FRAP test to study total value of oxidation compounds in the culture medium of cell. Finally DNA damages and oxidative reaction occurrence in control groups, under mild heat stress and under severe heat stress, were studied by experimental design (Random design) and statistical test (One way ANOVA) in statistical software (SPSS). The results of present study showed that by increasing temperature the rate of living cells decreases and the difference between living cells percent in control group 32 centigrade and the groups of 39 and 42 centigrade is significant (P