Use of RPMI in Vitreous Cytology: The Case for Sterile, Single Use Aliquots

Ru Jin Eugene Ting, Keller J,

Abstract

Importance: Sterile aliquots of Roswell Park Memorial Institute (RPMI) are necessary in obtaining uncontaminated vitreous biopsies. Background: To report a case of RPMI medium contamination in diagnostic pars plana vitrectomy (PPV) to exclude vitreoretinal lymphoma. Design: Interventional case report. Participants: One patient. Methods: Diagnostic pars plana vitrectomy, vitreous biopsy. Main outcome measures: Diagnostic, clinical, and histopathologic features of granulmoatous vitritis. Conclusion: A case for single use, sterile aliquots of refrigerated RPMI for vitreoretinal surgeons.