Nicotine has been characterized as a powerful parasympathomimetic alkaloid that collects in the roots and leaves of Nightshade group of plants. Point: This examination was planned for assessing the impacts of orally ingested nicotine in the histology of hippocampus, substantia nigra and cerebellum. Materials and Strategies: Twenty four grown-up male Wistar rodents (100g - 200g) were arbitrarily partitioned into 4 gatherings (bunch 1 - bunch 4). Gathering 1 filled in as the benchmark group, while bunches 2 - 4 were the rewarded gatherings. Nicotine was weakened in water and 1ml of the distinctive measurement (2mg/kg/day, 4mg/kg/day and 6mg/kg/day) were directed to the rewarded bunches individually with the guide of orogastric cannula for 42 days. Creatures were euthanized by cervical separation toward the finish of 7, 21 and 42 days in order to exhibit the portion and time dependant impact of this specialist. Cerebrum tissues were collected, handled and recolored utilizing Haematoxylin and eosin as indicated by standard histological procedures. Recolored tissue pictures were caught utilizing advanced micrometer eyepiece and cell check was resolved utilizing stereological procedure. Measurable examination: Information got were exposed to factual investigation with the utilization of factual bundle for sociologies.